directed mutagenesis meaning in English
定向突变
定向诱变
Examples
- Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases
研究人员已经利用蛋白质工程和定点突变技术来改变各种醇腈酶的活性位点和底物特异性等。 - The principles , operation and applications of site - directed mutagenesis , gene fusion technology , and post - translational modification methods were introduced emphatically
着重阐述了基因定点突变技术、基因融合技术和翻译修饰技术等新兴定点固定化技术的原理、特点和操作。 - The experimental procedure that begins with a cloned segment of dna , or a protein sequence , and uses this knowledge to introduce programmed mutations ( through directed mutagenesis ) back into the genome in order to investigate gene and protein function
在已知dna克隆片段或蛋白质序列上引导程序性的变异（通过直接诱变）并返回到基因组，来研究基因和蛋白质功能的实验方法。 - We obtained the full length gene of hbfgf coding sequence with pcr , and adjusted the g + c content according the software dnasisv2 . 5 , and replaced the cys78 and cys96 with serines by site - directed mutagenesis . 2 . sequence result suggested one of the recombinant is correctly synthezied and cloned
用pcr方法合成了hbfgf编码区全长，其中前20个氨基酸的g + c含量按暨南大学硕士学位论文：大肠杆菌表达重组hbfgf结构和功能优化摘要照计算机软件计算的结果进行了调整，第78和96位上的半眯氨酸被突变为丝氨酸。